树鼩的骨元和骨细胞穴微观结构超微影像的定性比较

doi:10.16359/j.cnki.cn11-1963/q.2020.0048

人类学学报 ›› 2020, Vol. 39 ›› Issue (04): 564-575.doi: 10.16359/j.cnki.cn11-1963/q.2020.0048

树鼩的骨元和骨细胞穴微观结构超微影像的定性比较

Vijaya Lakshmi Pavani MOLLI¹, Anubhav JAIN², 傅江南³, 吴英杰⁴^,⁵^,⁶, Jian Q.FENG¹^,^*(), 王谦¹^,^*()

1.德克萨斯州A&M大学牙医学院生物医学系, 达拉斯75246,德克萨斯州,美国
2.天普大学牙医学院, 费城 19140,宾夕法尼亚州,美国
3.暨南大学实验动物中心, 广州510632,中国
4.山东第一医科大学（山东省医学科学院）山东省实验动物中心,山东省立医院,济南250062,中国
5.西奈山医学院医学系内分泌,糖尿病和骨骼疾病科, 纽约10029,纽约州,美国
6.纽约大学牙医学院分子病理系,纽约10010,纽约州,美国

收稿日期:2020-07-30 修回日期:2020-09-22 出版日期:2020-11-15 发布日期:2020-11-18
通讯作者: Jian Q.FENG,王谦
基金资助:
NIH/NIDCR Grant: DE025014 to J.Q.F.

Osteons and osteocyte lacunae in belanger’s treeshrews (Tupaia belangeri chinensis) - A qualitative image comparative study

Vijaya Lakshmi Pavani MOLLI¹, Anubhav JAIN², FU Jiangnan³, WU Yingjie⁴^,⁵^,⁶, Jian Q. FENG¹^,^*(), WANG Qian¹^,^*()

1. Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, Texas 75246, U.S.A.
2. Kornberg School of Dentistry, Temple University, Philadelphia, Pennsylvania 19140, U.S.A.
3. Institute of Laboratory Animals, Jinan University, Guangzhou, China
4. Shandong Laboratory Animal Center, Shandong Provincial Hospital, Shandong First Medical
5. University & Shandong Academy of Medical Sciences, Jinan, Shandong 250062, China
6. Division of Endocrinology, Diabetes and Bone Disease, Department of Medicine, Icahn Mount Sinai School of Medicine, New York 10029, USA

Received:2020-07-30 Revised:2020-09-22 Online:2020-11-15 Published:2020-11-18
Contact: Jian Q. FENG,WANG Qian

摘要/Abstract

摘要：

近年来,由于树鼩与灵长类动物的亲缘关系,它们引起了人类发展和疾病研究的兴趣。在这项对树鼩,鼠,狗,狒狒和人类的骨骼超微形态的比较研究中,我们定性分析了骨骼的微观结构和形态,以评估树鼩对人类的亲近程度。在3只成年雄性树鼩 (滇西亚种) (Tupaia belangeri chinensis）的股骨中,使用荧光素异硫氰酸盐样品制备和染色共聚焦成像研究了皮质骨的骨元结构,并使用酸蚀刻SEM观察了骨细胞穴的形态。总体而言,树鼩中骨样形成物的密度和结构以及骨细胞穴的形态更像鼠, 与人类,狗和狒狒都明显不同。这些发现表明,尽管树鼩在系统发育上比鼠更接近人类,但它们的骨骼超微形态仍与鼠接近。这是除狗和狒狒之外,第一次对树鼩的骨元和骨细胞穴进行超微影像的研究。这个比较研究的结果丰富了我们对早期灵长类动物骨骼发育,适应性和进化的理解。未来有必要进行进一步的定量比较研究来表征树鼩骨骼的微观形态。

关键词: 骨组织学, 皮质骨, 非人灵长类

Abstract:

In recent years, treeshrews have gained interest among researchers in the study of human development and disease owed to their phylogenetic closeness to primates. In this comparative study among a mouse, dog, human, baboon, and treeshrews, bone microstructure and morphology were quantitatively analyzed to assess the closeness of treeshrews to humans. In the femurs of three adult male Belanger's treeshrews (Tupaia belangeri chinensis), the osteon structure of the cortical bone was studied using confocal imaging via Fluorescein Iso-Thio-Cyanate sample preparation and staining. Osteocyte lacunae morphology was visualized using acid-etched SEM. Overall, the density and structure of osteon-like formation as well as the morphology of osteocyte lacunae in Belanger's treeshrews bore greater resemblance to mice than humans. These findings indicate that although treeshrews are phylogenetically closer to humans than mice, their bone morphology and functionality are still close to those of mice. This scenario was the first time that osteons and osteocyte lacunae were visualized and characterized in addition to those in a dog and baboon, which enriches our understanding of bone development, adaptation, and evolution in early primates. Future quantitative comparative study is warranted to characterize the micromorphology of bone in treeshrews.

Key words: Bone tissue histology, Cortical bone, Non-human primate model

中图分类号:

Q915.87

Vijaya Lakshmi Pavani MOLLI, Anubhav JAIN, 傅江南, 吴英杰, Jian Q.FENG, 王谦. 树鼩的骨元和骨细胞穴微观结构超微影像的定性比较[J]. 人类学学报, 2020, 39(04): 564-575.

Vijaya Lakshmi Pavani MOLLI, Anubhav JAIN, FU Jiangnan, WU Yingjie, Jian Q. FENG, WANG Qian. Osteons and osteocyte lacunae in belanger’s treeshrews (Tupaia belangeri chinensis) - A qualitative image comparative study[J]. Acta Anthropologica Sinica, 2020, 39(04): 564-575.

图/表 2

Fig.1 Confocal images 1a-1b. Confocal imaging represents osteon-like formation in the tree shrews at 20x and63x magnifications; 2a-2b. Confocal imaging that also shows the spindle to oval morphology of osteocytes in the cortical bone of mouse femora at 20x and 63x magnifications; 2c~2d. Confocal imaging represents osteon-like formation in the mice at 20x and 63x magnifications; 3a-3b. Confocal imaging represents osteon formation in the dog sample at 20x and 63x magnifications. Confocal imaging also shows the spindle morphology of osteocytes in the cortical bone of dog femora; 4a-4b. Confocal imaging demonstrates osteon formation in the baboon at two 20x and 63x magnifications. Confocal imaging also shows the spindle morphology of osteocytes in the cortical bone of baboon femora; 5a-5b. Confocal imaging represents osteon formation in the human specimens at 20x and 63x magnifications. Confocal imaging also shows the spindle morphology of osteocytes in the cortical bone of human femora specimens

Fig.2 Acid-etched SEM images 6. Acid-etched SEM images show spindle-shaped osteocyte lacunae in cortical bone of a tree shrew femur at 6a 250x, 6b 500x, 6c 1000x, and 6d 2000x magnifications; 7. Acid-etched SEM images show spindle-shaped osteocyte lacunae in cortical bone of mouse femora at 7a 250x, 7b 500x, 7c 1000x, 7d 2000x magnifications; 8. Acid-etched SEM images show osteon formation in the cortical bone of a dog femur at 8a 250x, 8b 500x, and spindle-shaped osteocyte lacunae in cortical bone resting in their lacuna at 8c 1000x as well as 8d 2000x magnifications; 9. Acid-etched SEM images show osteon formation in the cortical bone of a baboon femur at 9a 250x, 9b 500x, and spindle-shaped osteocyte lacunae at 9c 1000x and 9d 2000x magnifications; 10. Acid-etched SEM images show osteon formation with well-developed Haversian canals and adjacent lacunae connected by the canalicular system in the cortical bone of a human femur at 10a 250x and 10b 500x; stellate-shaped osteocyte lacunae in cortical bone at 10c 1000x and 10d 2000x magnifications

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树鼩的骨元和骨细胞穴微观结构超微影像的定性比较

Osteons and osteocyte lacunae in belanger’s treeshrews (Tupaia belangeri chinensis) - A qualitative image comparative study

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