激光共聚焦显微镜在微痕定量分析中的应用综述

doi:10.16359/j.cnki.cn11-1963/q.2020.0068

摘要/Abstract

摘要：

微痕分析是石器、骨器、牙齿等工艺、功能研究的常用方法,但无论是高倍还是低倍等常用方法,受限于体式光学显微镜、扫描电镜等观察方法,微痕分析通常很难进行原位定量分析,因此微痕分析的定量化是目前学术界遇到的主要问题。近年来随着激光共聚焦显微镜（LSCM）在微痕分析中的应用,微痕定量化在西方学术界得到了新的实践和进展。本文主要介绍激光共聚焦显微镜的原理及其在微痕分析中的应用案例,以期促进微痕分析在中国的进一步发展。

关键词: 微痕, 激光共聚焦显微镜, 粗糙度, 定量

Abstract:

Microwear analysis is a common method for the study of crafts and functions of lithics, bone implements, teeth, etc. However, whether the high-power or low-power technique, it’s difficult to describe in-situ quantitative analysis limited by observation methods such as stereo optical microscope and SEM. So the quantification of microwear analysis is the key problem encountered by the academic circles at present. In recent years, the quantification of microwear analysis has obtained new practice and progress in Western academic with the application of laser scanning confocal(LSC) microscope. This article mainly introduces the principle of LSC microscope and its cases and prospects with new approach in the research of microwear, which expect to promote the further development of microwear analysis in China.

Key words: Microwear, Laser scanning confocal microscope, Roughness, Quantification

中图分类号:

K854

崔天兴, 宋炜玮. 激光共聚焦显微镜在微痕定量分析中的应用综述[J]. 人类学学报, 2022, 41(01): 180-192.

CUI Tianxing, SONG Weiwei. Application review of the laser scanning confocal microscope in quantitative analysis of microwears[J]. Acta Anthropologica Sinica, 2022, 41(01): 180-192.

图/表 6

图1 LSCM成像原理示意图（A）[13]及LSCM基本结构（B）[14]

Fig.1 Schematic diagram of LSCM (A)[13] imaging principle and (B)[14] basic structure

图2 卷尾猴 (A) 和吼猴 (B) 的牙齿表面微痕纹理分析[26] A. 卷尾猴Capuchin monkey; B.吼猴howler monkey。卷尾猴以硬、脆类食物为主,吼猴以韧性好的树叶为主食

Fig.2 Dental microwear texture analysis[26]

图3 加工5种不同材料及未使用燧石的磨损表面粗糙度（Rq）数据横坐标为磨损区域上小尺度（3.8um）的Rq均值,纵坐标为大尺度（10.1um）的Rq均值

Fig.3 Roughness (Rq) data of the wear surface of five different materials and unused flint

图4 加工5种不同材料及未使用燧石的表面 LSCM显微照片,100×物镜/These were produced using the LSCM with a 100× objective lens

Fig.4 LSCM micrographs showing examples of the ﬁve different wear types and the unused ?int

图5 Sr,Co及SRC的多尺度关系示例 Co是根据SRC左侧尺度上的Sr值进行计算

Fig.5 An example of multiple area scales relationship of Sr, Co and SRC

图6 F检验结果 A.加工木材Sr;B.加工皮革Sr;C.木材与皮革Sr比较;D. 3种加工材料测量的Sr与尺度的关系[35];水平线上方的曲线（x）表示,可以在95％的置信度水平上进行区分

Fig.6 F-Test results(MSR)

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