新疆和田、哈密和阿勒泰市居民指甲中氢、氧稳定同位素的比较

doi:10.16359/j.cnki.cn11-1963/q.2020.0079

人类学学报 ›› 2021, Vol. 40 ›› Issue (05): 888-894.doi: 10.16359/j.cnki.cn11-1963/q.2020.0079

新疆和田、哈密和阿勒泰市居民指甲中氢、氧稳定同位素的比较

何欣龙¹(), 梅宏成²(), 王继芬¹, 朱军², 郭鹏然³, 胡灿², 何亚¹, 龚晓晓⁴, 胡展纶⁵

1.中国人民公安大学,北京 102600
2.公安部物证鉴定中心,北京 100038
3.广东省测试分析研究所广东省化学危害应急检测技术重点实验室,广州 510070
4.新疆警察学院,乌鲁木齐 830013
5.吉林省长春市公安局经济技术开发区公安分局,长春 130000

收稿日期:2019-11-27 修回日期:2020-05-29 出版日期:2021-10-15 发布日期:2021-10-15
通讯作者: 梅宏成
作者简介:何欣龙,硕士研究生,主要从事刑事技术方面的研究。E-mail: 1078683050@qq.com
基金资助:
国家重点研发计划课题(2017YFC0803803);公安部技术研究计划项目(2016JSYJB10);公安部物证鉴定中心资助开放课题(2019NELKFKT09)

Comparison of stable isotopes of hydrogen and oxygen in fingernails of the residents in Hotan, Hami and Altai, Xinjiang

HE Xinlong¹(), MEI Hongcheng²(), WAMG Jifen¹, ZHU Jun², GUO Pengran³, HU Can², HE Ya¹, GONG Xiaoxiao⁴, HU Zhanlun⁵

1. People’s Public Security university of China, Beijing 102600
2. Institute of Forensic Science, Ministry of Public Security, Beijing 100038
3. Guangdong Institute of Analysis, Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangzhou 510070
4. Xinjiang police academy, Urumqi 830013
5. Public Security Branch of Economic and Technological Development Zone, Changchun City Public Security Bureau of Jilin Province, Changchun130000

Received:2019-11-27 Revised:2020-05-29 Online:2021-10-15 Published:2021-10-15
Contact: MEI Hongcheng

摘要/Abstract

摘要：

在法庭科学研究领域,指甲作为一种新型生物信息载体,在物证信息挖掘方面具有重要的价值。人生命活动中摄入的氢、氧主要来源于饮用水、摄入的食物和空气等,其中的氢、氧稳定同位素比值信息会被指甲角蛋白记录并保存下来,这些信息可以用于推断人的生活地域变化情况,在个体身份的排查工作中具有重要的作用。本文借助元素分析-稳定同位素比质谱技术（EA-IRMS）对新疆和田、哈密和阿勒泰3个城市常住居民指甲中氢、氧稳定同位素比值进行了分析,并借助判别分析模型对指甲样本的氢、氧稳定同位素比值进行了模式识别。结果表明,3个不同城市常住居民指甲氢、氧稳定同位素比值存在一定的差异;和田指甲样本δ²H值与哈密、阿勒泰有明显差异;哈密指甲样本δ²H值与阿勒泰重叠范围宽,差异较小;3个城市δ¹⁸O值分布均有重叠,无明显的差异。在判别模型中,通过判别函数f(x,y)=0.992x+0.046y和判别函数f(x,y)=-0.198x+1.009y对3个市共计85份样本进行判别分析,总体分类准确率为95.29 %,9个验证样本均实现了准确的归类和判定。本文研究表明,不同城市居民指甲中的氢、氧稳定同位素比值是存在一定差异的,采用EA-IRMS结合判别分析能初步实现不同城市居民的区分。

关键词: 氢、氧稳定同位素, 指甲, 溯源, 法庭科学

Abstract:

Nail is a kind of connective tissue with a thyroid structure on the back of fingertip. In the field of forensic science, nail, as a new biological information carrier, has a high application value. The hydrogen and oxygen stable isotopes in water would be continuously fixed by nail keratin and kept in the nail statically after the person ingested water, thus the temporal and spatial information of the person’s life were recorded, which is of great significance for the screening of individual identity. In this paper, the hydrogen and oxygen stable isotope in fingernails of residents in Khotan, Hami and Altay were analyzed by Elemental Analyses-Stable Isotope Ratio Mass Spectrometry, and the model was constructed by discriminant analysis to carry out the pattern recognition. The results showed that the ratios of hydrogen and oxygen stable isotopes in the fingernails of residents in three different cities were different. There was a significant difference between the δ²H value in Khotan residents and that in Hami and Altay. Hami and Altay had more overlapping of δ ²H value, but the difference was relatively small. The distribution of δ ¹⁸O value about three cities was overlapped without any significant difference. In the discriminant model, the difference between Khotan and Hami, Altay was very obvious, while difference between Hami and Altay was partly coincidence. Compared with DF₂ discriminant function, each sample was obviously distinct from each other under DF₁ discriminant function. The overall classification accuracy of 85 samples was 95.29 %, and the 9 verified samples were classified exactly, which was ideal. In conclusion, the combination of EA-IRMS and discriminant analysis can provide an accurate and effective method for the analysis of hydrogen and oxygen stable isotopes in fingernails of different urban residents, which has certain reference significance.

Key words: Hydrogen oxygen Stable Isotope, Nail, Traceability, Forensic Science

中图分类号:

Q989
DF795

何欣龙, 梅宏成, 王继芬, 朱军, 郭鹏然, 胡灿, 何亚, 龚晓晓, 胡展纶. 新疆和田、哈密和阿勒泰市居民指甲中氢、氧稳定同位素的比较[J]. 人类学学报, 2021, 40(05): 888-894.

HE Xinlong, MEI Hongcheng, WAMG Jifen, ZHU Jun, GUO Pengran, HU Can, HE Ya, GONG Xiaoxiao, HU Zhanlun. Comparison of stable isotopes of hydrogen and oxygen in fingernails of the residents in Hotan, Hami and Altai, Xinjiang[J]. Acta Anthropologica Sinica, 2021, 40(05): 888-894.

图/表 4

表1 和田、哈密和阿勒泰三市居民指甲样本中δ2H值和δ18O值信息

Tab.1 The details of hydrogen and oxygen stable isotopes in fingernails from three areas

地域	测量值	最小值	最大值	均值	标准差
阿勒泰	δ²H	-87.9‰	-77.1‰	-81.2‰	3.1
阿勒泰	δ¹⁸O	4.4‰	7.9‰	6.8‰	0.8
哈密	δ²H	-86.7‰	-73.1‰	-78.9‰	3.4
哈密	δ¹⁸O	6.9‰	9.1‰	8.3‰	0.5
和田	δ²H	-63.7‰	-52.1‰	-57.5‰	2.5
和田	δ¹⁸O	6.6‰	12.4‰	8.9‰	1.8

图1 新疆三地人指甲中δ2H值和δ18O值分布

Fig.1 δ2H and δ18O in fingernails from the three ares in Xinjiang

表2 85个训练集样本判别函数摘要

Tab.2 The abstract of discriminant function about 85 training samples

函数	DF1	DF2
方差贡献率	0.953	0.047
正则相关性	0.967	0.644
函数检验	1 到 2	2
Wilks’ Lambda	0.038	0.585
显著性	0	0

图2 和田、哈密和阿勒泰市样本判别分析图

Fig.2 The discriminant scatter diagram about Khotan, Hami and Altay

参考文献 25

[1]	Delphine C, Michel Y, Hugo N, et al. Nail analysis for the detection of drugs of abuse and pharmaceuticals: a review[J]. Forensic Toxicology, 2015, 33(1):12-36 doi: 10.1007/s11419-014-0258-1 URL
[2]	Krumbiegel F, Hastedt M, Westendorf L, et al. The use of nails as an alternative matrix for the long-term detection of previous drug intake: validation of sensitive UHPLC-MS/MS methods for the quantification of 76 substances and comparison of analytical results for drugs in nail and hair samples[J]. Forensic Science, Medicine, and Pathology, 2016, 12(4):416-434 pmid: 27515931
[3]	Irina S, Rachel F, Joni J, et al. Hecht. Mass Spectrometric Quantitation of Nicotine, Cotinine, and 4-(Methylnitrosamino)-1-(3-Pyridyl)- 1-Butanol in Human Toenails[J]. cancer epidemiology biomarkers & prevention, 2005, 15(12):2378-2383
[4]	林光辉. 稳定同位素生态学[M]. 北京: 高等教育出版社, 2013: 1-2
[5]	Tsui MTK, Liu S, Brasso R, et al. Controls of methylmercury bioaccumulation in forest floor food webs[J]. Environmental Science & Technology. 2019, 53(5):2434-2440 doi: 10.1021/acs.est.8b06053 URL
[6]	McCormack SA, Trebilco R, Melbourne-Thomas J, et al. Using stable isotope data to advance marine food web modelling[J]. Reviews in Fish Biology and Fisheries. 2019, 29(2):277-296 doi: 10.1007/s11160-019-09552-4
[7]	Macko SA, Engel MH, Andrusevich V, et al. Documenting the diet in ancient human populations through stable isotope analysis of hair[J]. Philosophical Transactions of the Royal Society B: Biological Sciences. 1999, 354(1379):65-76 doi: 10.1098/rstb.1999.0360 URL
[8]	Schoeninger MJ, Iwaniec UT, Nash LT. Ecological attributes recorded in stable isotope ratios of arboreal prosimian hair[J]. Oecologia, 1998, 113(2):222-230 doi: 10.1007/s004420050372 pmid: 28308201
[9]	Thompson AH, Chesson LA, Podlesak DW, et al. Stable isotope analysis of modern human hair collected from Asia (China, India, Mongolia, and Pakistan)[J]. American Journal of Physical Anthropology, 2010, 141(3):440-451 doi: 10.1002/ajpa.21162 pmid: 19918992
[10]	O’Brien DM, Wooller MJ. Tracking human travel using stable oxygen and hydrogen isotope analyses of hair and urine[J]. Rapid Communications in Mass Spectrometry, 2007, 21(15):2422-2430 doi: 10.1002/(ISSN)1097-0231 URL
[11]	王宁, 李素婷, 李宏飞, 等. 古骨胶原的氧同位素分析及其在先民迁徙研究中的应用[J]. 科学通报, 2015, 60(9):838-846
[12]	司艺, 李志鹏, 胡耀武, 等. 河南偃师二里头遗址动物骨胶原的H、O稳定同位素分析[J]. 第四纪研究, 2014, 34(1):196-203
[13]	梅宏成, 朱军, 权养科, 等. 稳定同位素检验推断生物物证供体的生活时空信息[J]. 刑事技术, 2016, 41(2):87-92
[14]	Dahl TW. Identifying remnants of early Earth[J]. Science, 2016, 352(6287):768-769 doi: 10.1126/science.aaf2482 URL
[15]	Wilson ST, Hawco NJ, Armbrust EV, et al. Kīlauea lava fuels phytoplankton bloom in the North Pacific Ocean[J]. Science, 2019, 365(6457):1040-1044 doi: 10.1126/science.aax4767 URL
[16]	Tacail T, Martin JE, Arnaud-Godet F, et al. Calcium isotopic patterns in enamel reflect different nursing behaviors among South African early hominins[J]. Science Advances, 2019, 5(8): eaax3250
[17]	Burton ZFM, Moldowan JM, Magoon LB, et al. Interpretation of source rock depositional environment and age from seep oil, east coast of New Zealand[J]. International Journal of Earth Sciences. 2019, 1908(4):1079-1091
[18]	Fraser I, Meier-Augenstein W, Kalin RM. The role of stable isotopes in human identification: a longitudinal study into the variability of isotopic signals in human hair and nails[J]. Rapid Communications in Mass Spectrometry, 2006, 20(7):1109-1116 pmid: 16521167
[19]	Fraser I, Meier-Augenstein W. Stable ²H isotope analysis of modern-day human hair and nails can aid forensic human identification[J]. Rapid Communications in Mass Spectrometry, 2007, 21(20):3279-3285 pmid: 17879389
[20]	Thompson AH, Chesson LA, Podlesak DW, et al. Stable isotope analysis of modern human hair collected from Asia (China, India, Mongolia, and Pakistan)[J]. American Journal of Physical Anthropology, 2010, 114(3):440-451
[21]	Mützel (Rauch) E, Lehn C, Peschel O, et al. Assignment of unknown persons to their geographical origin by determination of stable isotopes in hair samples[J]. International Journal of Legal Medicine, 2009, 123(1):35-40 doi: 10.1007/s00414-008-0286-7 URL
[22]	Meier-Augenstein W. Stable Isotope Forensics: Methods and Forensic Applications of Stable Isotope Analysis (Developments in Forensic Science)[M]. Edinburgh: Wiley, 2018: 1-343
[23]	李崇博, 褚宏宽, 宋玉, 等. 哈密盆地地下水系统划分及特征研究[J]. 新疆地质, 2016, 34(1):139-143
[24]	孙永, 易朝路, 刘金花, 等. 昆仑山木孜塔格地区冰川发育水汽来源探讨[J]. 地球环境学报, 2018, 9(4):383-391
[25]	He XL, Wang JF. Rapid and Nondestructive Forensic Identification of Tire Particles by Attenuated Total Reflectance - Fourier Transform Infrared Spectroscopy and Chemometrics[J]. Analytical letter, 2020, 53(5):714-734

新疆和田、哈密和阿勒泰市居民指甲中氢、氧稳定同位素的比较

Comparison of stable isotopes of hydrogen and oxygen in fingernails of the residents in Hotan, Hami and Altai, Xinjiang

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