中国4个民族mtDNAD环多态性研究

人类学学报 ›› 2000, Vol. 19 ›› Issue (02): 127-131.

中国4个民族mtDNAD环多态性研究

陈伟,陈峰,薛亚丽,杨焕杰,傅松滨,张贵寅,李璞

出版日期:2000-06-15 发布日期:2000-06-15

Study on the mtDNAD loop polymorphism of four ethnic groups in China

Chen Wei, Chen Feng, Xue Yali, Yan Huanjie, Fu Songbin, Zhang Guiyin, Li Pu

Online:2000-06-15 Published:2000-06-15

摘要/Abstract

摘要： 本文利用聚合酶链反应限制性片段长度多态性 (PCR- RFL P)方法 ,检测了 36名汉族、 30名达斡尔族、 32名鄂伦春族、 30名鄂温克族随机选择正常个体 mt DNA D环 465bp DNA片段多态性并加以比较。结果表明 ,mt DNA D环该片段的 RFLP分析共产生 2 7种限制性类型 ,计算出 4个民族的 mt DNA D环平均核苷酸歧异频率 ,并对 4个民族的亲缘关系进行了聚类分析。

关键词: mtDNA;D环;遗传多态

Abstract: The PCR-RFLP techniques were used to study the mtDNA D loop polymorphism in Han ( 36 testees) , Daur ( 30 testees) , Oroqen ( 32 testees) and Ewenki ( 30 testees) . All testees were randomly selected and healthy. The polymorphisms on 10 sites of a 465bp- long DN A fragment in mtDNA D loop were detected and compared among ethnic groups.
Altogether 27 mito types were found by PCR-RFLP. The mean nucleic acid substitute frequency on each site was: Han 0. 0291, Daur 0. 0541, Oroqen 0. 0477 and Ewenki 0. 0563, respectively.

Key words: mtDNA, D loop, Genetic polymorphism

陈伟,陈峰,薛亚丽,杨焕杰,傅松滨,张贵寅,李璞. 中国4个民族mtDNAD环多态性研究[J]. 人类学学报, 2000, 19(02): 127-131.

Chen Wei, Chen Feng, Xue Yali, Yan Huanjie, Fu Songbin, Zhang Guiyin, Li Pu. Study on the mtDNAD loop polymorphism of four ethnic groups in China[J]. Acta Anthropologica Sinica, 2000, 19(02): 127-131.