我国东北地区3个群体DYS390多态位点的遗传学研究

人类学学报 ›› 2000, Vol. 19 ›› Issue (04): 318-323.

我国东北地区3个群体DYS390多态位点的遗传学研究

滕百军,陈峰,傅松滨,张贵寅,李璞

出版日期:2000-12-15 发布日期:2000-12-15

The genetics study of Chinese Y chromosomal microsatellite locus DYS390

Teng Baijun, Chen Feng, Fu Songbin, Zhang Guiyin, Li Pu

Online:2000-12-15 Published:2000-12-15

摘要/Abstract

摘要： 目的　研究中国群体Y染色体微卫星位点 DYS390遗传多态性 ,可以用于追溯人类进化上的父系祖先 ,也可以为人类基因组和法医学等研究积累数据。方法　采用 PCR技术扩增微卫星DNA片段 ,再经变性凝胶电泳及银染方法 ,对我国东北地区汉族、蒙古族及朝鲜族 3个群体的1 0 2例男性个体的 DYS390位点的遗传多态性进行了研究。结果　除汉族群体发现 5种等位基因外 ,朝鲜族和蒙古族群体均检出 4种。在汉族群体中 ,我们检出 1例具223bp等位基因。等位基因频率分布在汉族、朝鲜族以211bp的频率为最高 ,分别为0.439和0.451 ;而蒙古族群体则以215bp的频率为最高 (0.433)。结论　 3个群体之间 DYS390位点等位基因频率无显著性差异 ( Fisher精确概率检验 :P=0.930 )。 3个群体中DYS390位点 5种等位基因的分化程度以223bp为最高 ,分化程度最低的为211bp。聚类分析表明3个群体的父系亲缘关系较为密切 ,其中以汉族与朝鲜族之间的遗传距离最近。

关键词: DYS390;Y染色体;微卫星DNA;遗传多态性

Abstract: Purpose The study of Chinese Y chromosomal micro satellite locus DYS390 are used to trace paternal lineage of human origins and evolution. The data in this study may be useful in human population genetics and forensic applications. Methods In this experiment, the distribution of a Y-chromosome specific polymorphic locus, DYS390, in 102 unrelated males of three ethnic groups in China w as studied by using PCR method follow ed by PAGE and silver staining . The three nationalities are Han in Northeast of our country, Korean in Yan Bian and Mongol in Inner Mongolia Municipalities. Results The results showed that 5 alleles were existed in Han population, 4 alleles were existed in Korean and Mongolian populations. Their highest allele frequencies were 0. 439 ( 211bp) in Han, 0. 451 ( 211bp) in Korean, and 0. 433 ( 215bp) in Mongolian population. Besides, one case of allele 223bp was found in Han population ( 1 /41) . Conclusion The allele frequency difference among three populations is insignificant ( Fisher 's Ex act Test: p= 0. 930) . Clustering analyses showed that paternal lineage is intimate among the three nationalities.

Key words: DYS390, Y-ch romosome, Microsa tellite DNA, Polymorphism (genetics)

滕百军,陈峰,傅松滨,张贵寅,李璞. 我国东北地区3个群体DYS390多态位点的遗传学研究[J]. 人类学学报, 2000, 19(04): 318-323.

Teng Baijun, Chen Feng, Fu Songbin, Zhang Guiyin, Li Pu. The genetics study of Chinese Y chromosomal microsatellite locus DYS390[J]. Acta Anthropologica Sinica, 2000, 19(04): 318-323.