中国东北汉族群体3个MiniSTR基因座的遗传多态性研究

人类学学报 ›› 2010, Vol. 29 ›› Issue (04): 437-444.

中国东北汉族群体3个MiniSTR基因座的遗传多态性研究

马威; 李岩; 徐飞;

出版日期:2010-12-15 发布日期:2010-12-15

Polymorphism study of the 3 MiniSTR loci of Han ethnic in the Northeast China

MA Wei; LI Yan; XU Fei

Online:2010-12-15 Published:2010-12-15

摘要/Abstract

摘要： 我们使用D10S1248、D14S1434和D22S1045三个miniSTR基因座对东北汉族群体的141名随机个体样本进行遗传多态性研究, 通过荧光标记多重PCR扩增, 310遗传分析仪电泳, 确定个体的基因型, 获得相应的群体遗传数据。对所得数据进行统计学分析。共检出25个等位基因, 56种基因型。3个基因座在所检测群体内均符合哈迪温伯格平衡, 具有较高的杂合度、个体识别率和非父排除率。累积个体识别率为0.99876, 累计非父排除率为0.89819。3个基因座在东北汉族均具有高度的遗传多态性,在群体遗传学和法医学研究中有一定的应用价值。

关键词: MiniSTR; 遗传多态性; 等位基因; 东北地区汉族; 人类学

Abstract: Polymorphisms of three selected miniSTR loci, D10S1248, D14S1434, and D22S1045, were investigated in 141 Han ethnic people from northeastern Chinese populations. Multiple fluorescent polymerase chain reaction (PCR) techniques were performed for the three loci in one tube per individual sample, with the genotypic information obtained using an ABI 310 Genetic Analyzer with capillary electrophoresis. A total of 25 alleles and 56 genotypes were detected in the 3 miniSTR loci, and their frequency distributions showed no significant deviations from the Hardy-Weinberg equilibrium. Each of the three miniSTR loci showed high levels of heterozygosity, discrimination power, and power of exclusion. The accumulated power of discrimination and exclusion for the three loci was 0. 99876 and 0. 89819, respectively. The results of this study indicate that a high level of genetic polymorphisms can be observed in Han ethnic Chinese of Northeastern China when using the three loci selected. This research would be helpful in future population studies, and in forensic analyses.

Key words: MiniSTR; Genetic polymorphisms; Allele; Han; Northeastern China

马威; 李岩; 徐飞;. 中国东北汉族群体3个MiniSTR基因座的遗传多态性研究[J]. 人类学学报, 2010, 29(04): 437-444.

MA Wei; LI Yan; XU Fei. Polymorphism study of the 3 MiniSTR loci of Han ethnic in the Northeast China[J]. Acta Anthropologica Sinica, 2010, 29(04): 437-444.