广西毛南族DXS7133、DXS8378、DXS6789和DXS7423基因座遗传多态性

人类学学报 ›› 2015, Vol. 34 ›› Issue (03): 397-403.

广西毛南族DXS7133、DXS8378、DXS6789和DXS7423基因座遗传多态性

周丽宁;龚继春;伦军;邓琼英;覃耀春

出版日期:2015-09-15 发布日期:2015-09-15

Genetic of DXS7133, DXS8378, DXS6789 and DXS7423 in the Maonan Ethnic Group from Guangxi Province

ZHOU Lining, GONG Jichun, LUN Jun, DENG Qiongying, QIN Yaochun

Online:2015-09-15 Published:2015-09-15

摘要/Abstract

摘要： 采用PCR-STR及基因分型技术,对广西毛南族167名(女57,男110)健康无关个体4个X-STR基因座(DXS7133、DXS8378、DXS6789和DXS7423)的遗传多态性进行研究。结果显示4个X-STR基因座分别检出4、5、9、3个等位基因和5、9、18、5种基因型,4个X-STR基因座女性的基因型频率分布均符合Hardy-Weinberg平衡定律(P>0.05)。群体遗传多态性指标为:多态信息含量(PIC)0.9611、男性个体识别率(DPmale)0.9771、女性个体识别率(DPfemale)0.9980、父-母-女三联体非父排除率(MECtrio)0.9611、父-女二联体非父排除率(MECduo)0.8821,显示上述4个X-STR基因座均具有较高多态性,在法医学个人识别、亲权鉴定及群体遗传学研究中有重要应用价值,同时也为人类群体遗传学、法医学等研究提供了广西毛南族群体X-STR基因座的基础数据,丰富了中华民族基因数据库。

关键词: X染色体;短串联重复序列;遗传多态性;遗传距离;广西毛南族

Abstract: To determine the genetic polymorphism of four X-STR loci among 167 unrelated individuals (57 female, 110 male) for Maonan groups from Guangxi Province, DXS7133, DXS8378, DXS6789 and DXS7423 were genotyped by multiplex PCR and Genscan. The numbers of alleles in the four X-STR loci were 4, 5, 9 and 3, respectively; the numbers of genotypes in the four loci were 5, 9, 18 and 5, respectively. The genotype frequencies in females were in accordance with Hardy-Weinberg equilibrium (P>0.05). The combined polymorphism information content (PIC) was 0.9611, power of discrimination (DP) was 0.9771 in males and 0.0.9980 in females, and the combined mean exclusion chance (MEC) was 0.0.9611 in the trio cases and 0.8821 in the duo cases. The 4 X-STR loci were highly polymorphic in Guangxi Maonan population and it is powerful for individual identification, paternity testing and population genetics. As well as the human population genetics and forensic science research offers Guangxi Maonan group X-STR loci of basic data, enrich the genetic database of the Chinese nation.

Key words: X-chromosome; Short tandem repeats; Genetic polymorphism; Genetic distance; Guangxi Maonan ethnic group

周丽宁;龚继春;伦军;邓琼英;覃耀春. 广西毛南族DXS7133、DXS8378、DXS6789和DXS7423基因座遗传多态性[J]. 人类学学报, 2015, 34(03): 397-403.

ZHOU Lining, GONG Jichun, LUN Jun, DENG Qiongying, QIN Yaochun. Genetic of DXS7133, DXS8378, DXS6789 and DXS7423 in the Maonan Ethnic Group from Guangxi Province[J]. Acta Anthropologica Sinica, 2015, 34(03): 397-403.