中国西藏拉托唐古墓地古代居民线粒体全基因组研究

doi:10.16359/j.cnki.cn11-1963/q.2020.0078

人类学学报 ›› 2021, Vol. 40 ›› Issue (01): 1-11.doi: 10.16359/j.cnki.cn11-1963/q.2020.0078

• 研究论文 • 下一篇

中国西藏拉托唐古墓地古代居民线粒体全基因组研究

丁曼雨¹^,²^,³(), 何伟⁴, 王恬怡¹^,²^,⁵, 夏格旺堆⁴, 张明¹^,²^,³, 曹鹏¹^,², 刘峰¹^,², 戴情燕¹^,², 付巧妹¹^,²^,³()

1.中国科学院古脊椎动物与古人类研究所,中国科学院脊椎动物演化与人类起源重点实验室,北京 100044
2.中国科学院生物演化与环境卓越创新中心,北京 100044
3.中国科学院大学,北京 100049
4.西藏文物保护研究所,拉萨 850000
5.西北大学文化遗产学院,西安 710000

收稿日期:2019-02-26 修回日期:2019-05-07 出版日期:2021-02-15 发布日期:2021-02-25
通讯作者: 付巧妹
作者简介:丁曼雨（1993-）,女,中国科学院古脊椎动物与古人类研究所硕士研究生,主要从事古DNA研究。Email: dingmanyu@ivpp.ac.cn
基金资助:
国家重点研发计划(2016YFE0203700);国家自然科学基金项目(41672021);国家自然科学基金项目(91731303);国家自然科学基金项目(41630102);中国科学院(QYZDB-SS W-DQC003);中国科学院(XDB26000000);中国科学院(XDB13040000)

A study of the mitochondrial genome of ancient inhabitants from the Latuotanggu cemetery, Tibet, China

DING Manyu¹^,²^,³(), HE Wei⁴, WANG Tianyi¹^,²^,⁵, Shargan Wangdue⁴, ZHANG Ming¹^,²^,³, CAO Peng¹^,², LIU Feng¹^,², DAI Qingyan¹^,², FU Qiaomei¹^,²^,³()

1. Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044
2. CAS Center for Excellence in Life and Paleoenvironment, Beijing 100044
3. University of Chinese Academy of Sciences, Beijing 100049
4. Tibet Cultural Relics Protection Institute, Lhasa 850000
5. College of Cultural Heritage, Northwest University, Xi’an 710000

Received:2019-02-26 Revised:2019-05-07 Online:2021-02-15 Published:2021-02-25
Contact: FU Qiaomei

摘要/Abstract

摘要：

目前青藏高原高海拔地区古DNA研究匮乏。拉托唐古墓地位于青藏高原西南高海拔区域,本文对该墓地出土距今约700年的人骨进行古DNA提取,捕获了高质量线粒体全基因组数据,结合东亚线粒体基因组数据库,运用遗传统计方法开展分析。研究结果表明,距今3000年以内青藏高原西南部人群的遗传历史具有连续性,距今700年左右的拉托唐古墓地居民与距今3150-1250年的古代尼泊尔居民以及现代中国西藏居民母系遗传关系较近,且他们都具有共同的M9a1a1c1b1a单倍群。对M9a1a1c1b1a单倍群的深入研究发现,距今10930-5150年期间青藏高原可能发生了人口扩张事件。以上结果为我们了解古代青藏高原高海拔地区人群遗传历史提供了重要信息。

关键词: 青藏高原, 古DNA, 线粒体全基因组, 拉托唐古, 新石器时代

Abstract:

With rapid advances in next generation sequencing technologies, we have extracted three ancient DNA from samples from Tibet. With a dataset of present-day East Asians, we tried to reconstruct the history of this region using population genetic methods. Using the mtDNA genome, our study focused on the maternal relationship between ancient Tibetans living in 700BP and present-day populations, which revealed a genetic continuity on Tibetan plateau between the LaTuoTangGu (LTTG) cemetery and present-day Tibetans. This study is the first mtDNA genomic research of high elevation Tibetans.

Key words: Tibet, Ancient DNA, Mitochondrial genome, Latuotanggu, Neolithic

中图分类号:

Q987

丁曼雨, 何伟, 王恬怡, 夏格旺堆, 张明, 曹鹏, 刘峰, 戴情燕, 付巧妹. 中国西藏拉托唐古墓地古代居民线粒体全基因组研究[J]. 人类学学报, 2021, 40(01): 1-11.

DING Manyu, HE Wei, WANG Tianyi, Shargan Wangdue, ZHANG Ming, CAO Peng, LIU Feng, DAI Qingyan, FU Qiaomei. A study of the mitochondrial genome of ancient inhabitants from the Latuotanggu cemetery, Tibet, China[J]. Acta Anthropologica Sinica, 2021, 40(01): 1-11.

图/表 6

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建库号Library ID	样本号 Sample ID	墓号 Cemetery ID	平均污染度 Average Contamination	污染95%置信区间 95% CI of Contamination	5’ C->T	测年 ¹⁴C Date(BP)	覆盖度 Coverage	单倍体型Haplotype
L7056	C3425	M4	0.04%	0.01-0.13	2.2 %	760~675	476.6	M9a1a1c1b1a
L7055	C3427	M6	0.76%	0.55-1.05	9 %	NA	372.0	M9a1a1c1b1a
L7054	C3428	M5②	0.69%	0.48-0.99	3.9 %	NA	396.1	A17

建库号Library ID	样本号 Sample ID	墓号 Cemetery ID	平均污染度 Average Contamination	污染95%置信区间 95% CI of Contamination	5’ C->T	测年 ¹⁴C Date(BP)	覆盖度 Coverage	单倍体型Haplotype
L7056	C3425	M4	0.04%	0.01-0.13	2.2 %	760~675	476.6	M9a1a1c1b1a
L7055	C3427	M6	0.76%	0.55-1.05	9 %	NA	372.0	M9a1a1c1b1a
L7054	C3428	M5②	0.69%	0.48-0.99	3.9 %	NA	396.1	A17

地区 Area	人群 Population	M9a Frequency	文献来源 Reference
中国西藏	LTTG	50%	本研究
尼泊尔	a.Nepal	50%	[6]
中国西藏	Nyingchi	13%	[9]
中国西藏	Lhasa	25%	[9]
中国西藏	Shigatse	25%	[9]
中国西藏	Chamdo	13%	[9]
中国西藏	Deng	39.56%	[13]
中国西藏	Monpa	41.17%	[13]
中国西藏	Lhoba	15.38%	[13]
中国西藏	Sherpa	18.40%	[13]
中国西藏	Ngari	24%	[9]
中国北方	Daur	10%	[8]
中国北方	Tu	10%	[8]
中国南方	Han	2%	[8]
尼泊尔	Nepal	12%	[19]
印度北部	NE.India	9%	[15]

地区 Area	人群 Population	M9a Frequency	文献来源 Reference
中国西藏	LTTG	50%	本研究
尼泊尔	a.Nepal	50%	[6]
中国西藏	Nyingchi	13%	[9]
中国西藏	Lhasa	25%	[9]
中国西藏	Shigatse	25%	[9]
中国西藏	Chamdo	13%	[9]
中国西藏	Deng	39.56%	[13]
中国西藏	Monpa	41.17%	[13]
中国西藏	Lhoba	15.38%	[13]
中国西藏	Sherpa	18.40%	[13]
中国西藏	Ngari	24%	[9]
中国北方	Daur	10%	[8]
中国北方	Tu	10%	[8]
中国南方	Han	2%	[8]
尼泊尔	Nepal	12%	[19]
印度北部	NE.India	9%	[15]

中国西藏拉托唐古墓地古代居民线粒体全基因组研究

A study of the mitochondrial genome of ancient inhabitants from the Latuotanggu cemetery, Tibet, China

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