中国人群中微卫星位点DXYS156的多态研究

人类学学报 ›› 2000, Vol. 19 ›› Issue (02): 121-126.

中国人群中微卫星位点DXYS156的多态研究

许丽萍,Michael F.Hammer,Tatiana Karafet,杜若甫

出版日期:2000-06-15 发布日期:2000-06-15

A study on polymorphism of microsatellite locus DXYS156 in Chinese populations

Xu Liping, Michael F. Hammer, Tatiana Karafet, Du Ruofu

Online:2000-06-15 Published:2000-06-15

摘要/Abstract

摘要： 以中国 2个汉族群体和 8个少数民族群体的 52 0名个体为研究对象 ,采用 PCR扩增后聚丙烯酰胺凝胶电泳分离的方法 ,分析了 Y染色体上 DXYS1 56Y和 X染色体上 DXYS1 56X这两个微卫星位点的遗传多态性。结果表明 :在所研究的10个中国人群中 ,共观察到10个不同长度片段的等位基因 ,在 X染色体上的 5个等位基因是 :130bp、 135bp、 140bp、 145bp、 150bp,在Y染色体上的五个等位基因是 :160 bp、 165bp、 170 bp、 175bp、 180 bp。 DXYS1 56Y位点的多态信息量较多 ,以 1 65bp为 Y染色体上的主要等位基因 ,频率范围在 0 .23— 0 .77;等位基因160bp、 170bp和175bp次之 ,频率范围分别为 0.0 2— 0.36、 0 .01— 0 .50和 0 .03— 0 .31 ;而等位基因 180 bp频率较低 ,频率范围为 0.02— 0.05。 DXYS1 56X位点的多态信息量较少 ,以等位基因140bp为各个群体中最普遍的类型 ,频率范围为0.86— 0.98,其余类型均较低。DXYS1 56X的基因频率及中国人群中 DXYS1 56Y的基因频率至今在文献中尚未见报道。

关键词: 中国人;微卫星DNA;DXYS156;遗传多态性

Abstract: The genetic polymorphisms of micro satellite loci, DXYS156Y on the Y chromosome and DXYS156X on the X chromosome were studied by using PCR method follow ed by PAGE, in 520 males of 10 populations ( 2 Han subpopulations and 8 ethnic minorities) in China. The result shows that the ten alleles with different length fragments were found in Chinese populations surveyed. Among them, five are alleles 130bp, 135bp, 140bp, 145bp, 150bp on the X chromosome, and the others are alleles 160bp, 165bp, 170bp, 175bp, 180bp on the Y chromosome. The locus DXYS156Y has a high polymorphic information content, and the allele 165bp with the frequency range from 0. 23— 0. 77 is predominant in Chinese populations, followed by alleles 160bp, 170bp, and 175bp with the frequency range from 0. 02— 0. 36, 0. 01— 0. 50 and 0. 03— 0. 31, respectively, while the allele 180bp takes the last place with the frequency range from 0. 02— 0. 05. The DXYS156X has a low information content, and the allele 140bp, with the frequency range from 0. 86— 0. 98, is the most common type, while the other alleles have a quite low frequencies. The allele frequency of DXYS156Y in Chinese populations and that of DXYS156X in human populations have not been reported so far.

Key words: Chinese, Microsa tellite DNA, DXYS156, Genetic polymorphism

许丽萍,Michael F.Hammer,Tatiana Karafet,杜若甫. 中国人群中微卫星位点DXYS156的多态研究[J]. 人类学学报, 2000, 19(02): 121-126.

Xu Liping, Michael F. Hammer, Tatiana Karafet, Du Ruofu. A study on polymorphism of microsatellite locus DXYS156 in Chinese populations[J]. Acta Anthropologica Sinica, 2000, 19(02): 121-126.