云南白族人群Y染色体DYF155S1基因座多态性研究

人类学学报 ›› 2004, Vol. 23 ›› Issue (03): 239-247.

云南白族人群Y染色体DYF155S1基因座多态性研究

黄艳梅,伍新尧,蔡贵庆,童大跃,区敬华,曾艳红

出版日期:2004-09-15 发布日期:2004-09-15

Study on the polymorphism of dyf155s1 locus, Y-specific minisatellite, among Bai population of Yunnan province

HUANG Yan-mei; WU Xin-yao; CAI Gui-qing; TONG Da-yue; OU Jing-hua; ZENG Yan-hong

Online:2004-09-15 Published:2004-09-15

摘要/Abstract

摘要： 揭示云南白族136例无关男性个体Y染色体小卫星DYF155S1基因座多态性。用已建立的AmpFLP和MVRPCR方法分析DYF155S1基因座长度多态性、5′端多态性、3′端多态性。DYF155S1基因座具有高度多态性，基因多样性（h）达09996。研究表明将AmpFLP和荧光MVRPCR结合起来更能充分揭示DYF155S1基因座多态性，可为遗传学、人类学及法医学的研究提供有效的工具和基础资料。

关键词: DYF155S1;MVR-PCR;Y染色体;人类学;遗传学

Abstract: This study is to reveal the polymorphism of DYF155S1 locus, Y-specific minisatellite, among 136 cases unrelated male in Bai population of Yunnan Province.The analytical methods include Amp-FLP (Amplified fragment length polymorphism) and MVR-PCR (minisatellite variant repeat by PCR) with type 1-specific primer at 5′end and type 4-specific primer at 3′end. It is found that DYF155S1 locus possesses a high polymorphism and its gene diversity (h) reaches 0. 9996. These results indicated that Amp-FLP in combination with MVR-PCR may reveal fully the polymorphism of DYF155S1 locus and provide a useful tools and more data for the field of human genetics, anthropology and forensic medicine.

Key words: DYF155S1; MVR-PCR; Y chromosome; Anthropology; Genetics

黄艳梅,伍新尧,蔡贵庆,童大跃,区敬华,曾艳红. 云南白族人群Y染色体DYF155S1基因座多态性研究[J]. 人类学学报, 2004, 23(03): 239-247.

HUANG Yan-mei; WU Xin-yao; CAI Gui-qing; TONG Da-yue; OU Jing-hua; ZENG Yan-hong. Study on the polymorphism of dyf155s1 locus, Y-specific minisatellite, among Bai population of Yunnan province[J]. Acta Anthropologica Sinica, 2004, 23(03): 239-247.