广西环江县毛南族九个短串联重复序列基因座的遗传学分析

人类学学报 ›› 2007, Vol. 26 ›› Issue (03): 264-269.

广西环江县毛南族九个短串联重复序列基因座的遗传学分析

周丽宁;李松峰;徐林;龚继春;邓琼英

出版日期:2007-09-15 发布日期:2007-09-15

A study of polymorphisms of 9 STR Loci in the Maonan ethnic group of Huanjian, Guangxi Province

ZHOU Lining, LI Songfeng, XU Lin, GONG Jichun, DENG Qiongying

Online:2007-09-15 Published:2007-09-15

摘要/Abstract

摘要： 为了了解广西环江毛南族人群无关个体的九个短串联重复序列:vWA,D18S51,D5S818,FGA,D8S1179,D21S11,D7S820,D3S1358,D13S317基因座的遗传多态性分布情况;本文用枸橼酸钠抗凝法采集广西环江县毛南族200份无亲缘关系的健康个体的血样,Chelex-100方法提取DNA,应用AmpFlSTRIdentifilerTM荧光标记复合扩增技术对血样DNA的九个STR基因座进行扩增,用ABI 3100型遗传分析仪对扩增产物进行检测。结果显示九个STR位点的基因型分布均符合Hardy-Weinberg平衡定律,累积非父排除率达0.999996,累积个体识别能力达0.99999999996,多态信息总量为0.9999985。结论:广西环江县毛南族人群有自身的STR等位基因分布特征,所获数据可为法医学个体识别、亲子鉴定及群体的遗传学研究提供依据。

关键词: STR;基因频率;遗传距离;毛南族

Abstract: To investigate the distribution of nine short tandem repeat (STR) loci in the Maonan ethnic group from Guangxi Huanjiang. We collected the sodium citratedblood specimens from 200 healthy unrelated Maonan individuals in Guangxi Huanjiang, and used the Chelex100 method to extracte DNA by using AmpFSTR IdentifilerTM PCR amplification kit and 3100 genetic analyzer. In the study, a total of 37 alleles and 170 genotypes of the nine shorttandem repeat loci were found in 200 specimens. The allele frequency and genotype frequency were 0.0025-0.4600 and 0.0050-0.2300, respectively. The expected distribution of this genotype accords with HardyWeinberg equilibrium(P > 0.05) . The total discrimination power (TDP), total polymorphism information (TPI), cumulative paternity exclusion power(ECP) and average heterozygosity were 0.99999999996, 0.9999985, 0.999996 and 0.8028, respectively. These results demonstrate that these nine STR loci are of high polymorphic value and hereditary stability, and are in accord with Mendel# s law. The data obtained are valuable for rearch in population genetics research, forensic application, and individual identifications.

Key words: Short tandem repeat; Gene frequency; Genetic distance; Maonan Ethnic Group

周丽宁;李松峰;徐林;龚继春;邓琼英. 广西环江县毛南族九个短串联重复序列基因座的遗传学分析[J]. 人类学学报, 2007, 26(03): 264-269.

ZHOU Lining, LI Songfeng, XU Lin, GONG Jichun, DENG Qiongying. A study of polymorphisms of 9 STR Loci in the Maonan ethnic group of Huanjian, Guangxi Province[J]. Acta Anthropologica Sinica, 2007, 26(03): 264-269.