曲阜地区孔姓人群17个Y-STR基因座遗传多态性分析

人类学学报 ›› 2016, Vol. 35 ›› Issue (01): 125-131.

曲阜地区孔姓人群17个Y-STR基因座遗传多态性分析

侯伟光;王传超;蒋世洪;刘海东;李辉

出版日期:2016-03-15 发布日期:2016-03-15

Genetic Diversity of Seventeen Y-STR Loci among the People with the Surname Kong from Qufu Prefecture

HOU Weiguang, WANG Chuanchao, JIANG Shihong, LIU Haidong, LI Hui

Online:2016-03-15 Published:2016-03-15

摘要/Abstract

摘要： 应用AmpFLSTR~Y-filer~(TM)PCR Amplification Kit荧光标记复合扩增试剂盒(ABI公司),对曲阜地区11 18名孔姓男性个体血样DNA进行PCR,扩增,统计分析17个Y-STR基因座的遗传学参数。实验结果显示,17个基因座除DYS385a/b基因座检出51个单倍型外,其余基因座上分别检出4~11种等位基因,等位基因频率分布在0.0009~0.8265之间。由17个基因座组成的YH单倍型系统共检出206种单倍型。根据Y-STR单倍型推断了Y-SNP单倍群,发现曲阜孔姓有3种高频单倍群:C3、Q1a1和O3,前两者有着明显的单祖先扩散结构,最可能是孔子类型。本实验通过对曲阜地区孔姓人群群体17个Y染色体短串联重复序列基因座遗传多态性的调查,记录、保存孔姓人群遗传学数据。

关键词: 群体遗传学;曲阜地区;孔姓人群;Y染色体短串联重复序列

Abstract: Blood samples were collected from 1118 male individuals of Kong clans in the Qufu Prefecture of Shandong Province. Seventeen Y- chromosomal short tandem repeats (STR) loci were amplified in one tube by using the AmpFLSTR? Y-filerTM PCR Amplification Kit for each person, and then the frequency of alleles and genotypes of group was obtained. The results show that, except DYS385a/b locus was detected 51 haplotypes, the rest loci were detected from 4 to 11 alleles, the allele frequency distribute from 0.0009 to 0.8265. Totally, 206 haplotypes were detected in the YH haplotype system consisting of 17 loci. According to the Y-STR haplotypes, we inferred the Y-SNP haplogroups. We found three high frequency haplogroups among the Kong clans, i.e., C3, Q1a1, and O3. Both C3 and Q1a1 exhibited a mono-ancestor diffusion structure, indicating that either of them may be the haplogroup of Confucius. By investigating the 17 Y- STR loci among the Kong clans from Qufu Prefecture, we recorded the basic date of Kongpopulation.

Key words: Population Genetics; Kong surname; QufuPrefecture; Y chromosomal short tandem repeat

侯伟光;王传超;蒋世洪;刘海东;李辉. 曲阜地区孔姓人群17个Y-STR基因座遗传多态性分析[J]. 人类学学报, 2016, 35(01): 125-131.

HOU Weiguang, WANG Chuanchao, JIANG Shihong, LIU Haidong, LI Hui. Genetic Diversity of Seventeen Y-STR Loci among the People with the Surname Kong from Qufu Prefecture[J]. Acta Anthropologica Sinica, 2016, 35(01): 125-131.