新石器时代人骨遗骸中古代DNA的提取及X-Y染色体同源基因片段的PCR扩增

人类学学报 ›› 1996, Vol. 15 ›› Issue (03): 200-209.

新石器时代人骨遗骸中古代DNA的提取及X-Y染色体同源基因片段的PCR扩增

赵凌霞

出版日期:1996-09-15 发布日期:1996-09-15

Ancient DNA extraction from Neolithic human skeletal remains and PCR based amplification of the X-Y homologous amelogenin gene

Zhao Lingxia, Susanne Hummel, Cadja Lassen et al.

Online:1996-09-15 Published:1996-09-15

摘要/Abstract

摘要： 从中国新石器时代人骨遗骸中提取出古代DNA。通过聚合酶链式反应(PCR)技术扩增得到X-Y染色体上的单拷贝同源基因片段(Amelogenin Gene)。由于扩增的基因片段长度具有性别多态性,从而为古代人骨和牙齿提供了分子生物学的性别鉴定。

关键词: 古代DNA;Amelogenin基因;聚合酶链式反应;性别鉴定

Abstract: Ancient DNA was extracted from Neolithic human skeletal remains excavated in China. Single copy sequences of the X- Y homologous Amelogenin gene were amplified from the DNA extracts by PCR technique. Since the amplified sequences exhibit a sex specific length dimorphism they enable a sex identification on a molecular basis for ancient human bone and teeth.

Key words: Ancient DNA; Amelogenin gene; PCR; Sex identification

赵凌霞. 新石器时代人骨遗骸中古代DNA的提取及X-Y染色体同源基因片段的PCR扩增[J]. 人类学学报, 1996, 15(03): 200-209.

Zhao Lingxia, Susanne Hummel, Cadja Lassen et al.. Ancient DNA extraction from Neolithic human skeletal remains and PCR based amplification of the X-Y homologous amelogenin gene[J]. Acta Anthropologica Sinica, 1996, 15(03): 200-209.