江浙沪和哈尔滨地区汉族D17S30位点的多态性分布

人类学学报 ›› 1999, Vol. 18 ›› Issue (01): 53-59.

江浙沪和哈尔滨地区汉族D17S30位点的多态性分布

冯哲,冯明亮,嵇月华,张雁征,杨颖,陆琼,张工梁,杜庆田,陈仁彪

出版日期:1999-03-15 发布日期:1999-03-15

Distribution of D17S30 locus polymorphism of Chinese Hans from Jiangsu-Zhejiang-Shanghai and Harbin

Feng Zhe, Feng Mingliang, Ji Yuehua, Zhang Yanzheng, Yang Ying, Lu Qiong, Zhang Gongliang, Du Qingtian, Chen Renbiao

Online:1999-03-15 Published:1999-03-15

摘要/Abstract

摘要： D17S30是位于人类染色体17p13.3、以70bp为重复单位的、具有高度多态性的VNTR位点。本文采用作者报道的微量快速Amp-FLP分型技术，对100名哈尔滨市北方汉族人和110名江浙沪南方汉族人作了D17S30位点分型，发现在北方汉族与江浙沪南方汉族之间等位基因频率分布并无显著差异，但可见A1和A7基因频率北高南低，A4基因频率则为南高北低，提示存在南北汉族之间的分化。D17S30位点南北方汉族观察杂合性均为0.88，个体鉴别力均为0.97，多态性信息含量均为0.86。本文结果提示，采用微量快速Amp-FLP分型技术分析D17S30位点多态性，具有较高的灵敏度与准确性，可在我国作为一种高效的遗传标记应用于民族演变、法医学个体指认与亲子鉴定、遗传病基因连锁分析等研究领域。

关键词: D17S30位点;微量快速Amp－FLP;VNTR

Abstract: D17S30 is a highly polymorphic variable number of tandem repeats ( VNTR) locus with repeat unit being a segment of 70bp, which is located on human chromosome 17p13. 3. Using a miniature and rapid amplified fragment length polymorphism ( Amp- FLP) technique reported by this paper 's authors, we typed the D17S30 loci in 100 northern Chinese Hans from Harbin and 110 southern Chinese Hans from Jiangsu-Zhejiang-Shanghai areas, and discovered that there is no significant difference of D17S30 allele frequencies between northern and southern Hans with A1 and A7 having frequencies higher in northerners and lower in southerners, and with A4 vice versa suggesting differentiation between northern and southern Chinese Hans. The observed h eterozygosity , power of discrimination and polymorphism information content of the D17S30 locus are 0. 88, 0. 97 and 0. 86 respectively for both northern and southern Hans. The results obstained indicate that analysis of D17S30 locus polymorphism with the miniature and rapid Amp- FLP technique has higher sensitivity and accuracy and can be used as an efficient genetic markers in the studies of ethnic evolution, individual recognition and paternity test in forensic diagnosis, and linkage analysis of genetic disease genes.

Key words: D17S30 locus, Miniature and rapid Amp-FLP, VNTR

冯哲,冯明亮,嵇月华,张雁征,杨颖,陆琼,张工梁,杜庆田,陈仁彪. 江浙沪和哈尔滨地区汉族D17S30位点的多态性分布[J]. 人类学学报, 1999, 18(01): 53-59.

Feng Zhe, Feng Mingliang, Ji Yuehua, Zhang Yanzheng, Yang Ying, Lu Qiong, Zhang Gongliang, Du Qingtian, Chen Renbiao. Distribution of D17S30 locus polymorphism of Chinese Hans from Jiangsu-Zhejiang-Shanghai and Harbin[J]. Acta Anthropologica Sinica, 1999, 18(01): 53-59.