Genetic Polymorphism of 13 RM Y-STR Loci in Shandong Population

doi:10.16359/j.cnki.cn11-1963/q.2017.0073

Abstract

Abstract:

To verify the allele frequency and the haplotype diversity of the 13 rapidly mutating (RM) Y-STRs in Shandong province, Chinese Han population, we collected 154 unrelated male blood samples on FTA cards, and amplified the 13 RM Y-STRs with multiple PCR and tested the genotype using 3130XL (ABI) Genetic Analyzer. Among 13 RM Y-STRs, we observed a total of 331 alleles, and the values of gene diversity for each STR ranged from 0.7643 (DYS576) ~ 0.9946 (DYF399S1abc). Altogether 154 different haplotypes were identified from 154 unrelated male individuals and the haplotype diversity reached 1. We make a Conclusion that 13 RM Y-STRs show strong discrimination power and great application value for forensic in Shandong Han population.

Key words: Rapidly mutating, Genetic polymorphic, Haplotype, Shandong, Y-chromosome

CLC Number:

Q987

CHEN Yuling, LI Min, ZHAO Zhenmin, Li Yanan, BAO Yun, ZHU Ruxin, SHENG Xiang, WANG Yali, MA Xingyuan. Genetic Polymorphism of 13 RM Y-STR Loci in Shandong Population[J]. Acta Anthropologica Sinica, 2019, 38(02): 292-303.

Figures/Tables 5

References 23

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DYS626		DYF403S1b1		DYS404S1ab		DYF387S1ab		DYF403S1a
等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率
26	1.30%	15.2	2.60%	12-13 13	5.20%	34	0.60%	32-34	0.60%
27	2.60%	16.2	9.70%	12-14	0.60%	34.2-38	0.60%	34-36	0.60%
28	19.50%	17.2	18.20%	12-15	1.30%	34-37	2.60%	34-39	1.30%
29	22.10%	18.2	20.80%	13	18.20%	34-38	1.30%	35.2-42	0.60%
30	16.20%	19.2	18.20%	13-14	20.10%	34-39	3.20%	35-37	0.60%
31	19.50%	20.2	12.30%	13-15	5.80%	34-41	0.60%	35-38	1.90%
32	7.10%	21.2	11.00%	13-16	4.50%	35	1.30%	35-39	1.30%
33	5.80%	22.2	4.50%	14	9.10%	35-37	1.90%	35-41	0.60%
34	5.20%	23.2	1.90%	14-15	7.80%	35-38	14.30%	35-42	0.60%
35	0.60%	24.2	0.60%	14-15-16	0.60%	35-39	7.10%	36	1.30%
DYS449		DYF403S1b2		14-16	3.90%	35-40	0.60%	36-37	1.90%
等位基因	频率	等位基因	频率	14-17	1.90%	36	5.20%	36-38	3.90%
26	1.90%	51.2	2.60%	14-18	0.60%	36-37	6.50%	36-40	1.30%
27	4.50%	52.2	3.20%	15	10.40%	36-38	9.10%	36-42	0.60%
28	7.80%	53.2	2.60%	15-16	5.20%	36-39	5.20%	36-43	0.60%
29	9.70%	54.2	1.30%	15-16-17	0.60%	36-40	3.20%	36-45	0.60%
30	10.40%	55.2	3.90%	16	1.30%	36-41	1.30%	37	1.30%
31	16.20%	56.2	7.80%	16	1.30%	36-42	0.60%	37-38	7.10%
32	13.00%	57.2	9.10%	16-17	1.90%	37	2.60%	37-39	1.90%
33	13.60%	58.2	14.90%	17	0.60%	37-38	8.40%	37-40	2.60%
34	13.60%	59.2	14.30%	DYS526a		37-39	1.90%	37-41	1.30%
35	6.50%	60.2	15.60%	等位基因	频率	37-40	1.30%	37-42	1.90%
36	1.30%	61.2	13.60%	12	11.00%	37-41	0.60%	37-43	2.60%
37	0.60%	62.2	5.80%	13	20.10%	38	5.20%	38	4.50%
38	0.60%	63.2	2.60%	14	29.20%	38-39	1.30%	38.2-41	0.60%
DYS627		64.2	2.60%	15	28.60%	38-40	3.90%	38-39	5.20%
等位基因	频率	DYS612		16	10.40%	38-41	0.60%	38-40	5.20%
16	1.30%	等位基因	频率	17	0.60%	38-42	0.60%	38-41	3.20%
17	3.20%	31	1.30%	DYS526b		39	1.90%	38-42	1.30%
18	15.60%	32	4.50%	等位基因	频率	39-40	3.20%	39	1.90%
19	13.60%	33	4.50%	33	0.60%	40	1.90%	39-40	8.40%
20	20.80%	34	9.70%	34	5.20%	40-41	0.60%	39-41	5.20%
21	16.20%	35	9.70%	35	11.00%	DYS518		39-42	1.90%
21.2	0.60%	36	18.80%	36	15.60%	等位基因	频率	40	5.80%
22	16.90%	37	18.20%	37	33.80%	33	1.30%	40-41	4.50%
23	6.50%	38	12.30%	38	21.40%	34	3.20%	40-43	1.90%
24	4.50%	39	17.50%	39	9.70%	35	12.30%	40-44	0.60%
25	0.60%	40	3.20%	40	2.60%	36	24.70%	41	1.30%
DYS576		DYS547		DYS570		36.2	0.60%	41-42	0.60%
等位基因	频率	等位基因	频率	等位基因	频率	37	16.90%	41-43	3.20%
10	0.60%	42	3.20%	14	2.60%	37.2	0.60%	41-44	0.60%
14	0.60%	43	2.60%	15	4.50%	38	14.90%	41-46	0.60%
15	1.30%	44	3.20%	16	20.80%	39	14.30%	42	1.30%
16	7.80%	45	5.20%	17	21.40%	40	3.90%	42-43	1.30%
17	15.60%	46	7.10%	18	23.40%	41	4.50%	42-44	1.30%
18	39.60%	47	16.90%	19	20.80%	42	0.60%	42-46	0.60%
19	20.10%	48	26.00%	20	5.20%	43	0.60%	43-45	0.60%
20	11.00%	49	14.30%	21	1.30%	44	0.60%
21	2.60%	50	13.00%			46	0.60%
22	0.60%	51	3.90%
		52	4.50%
DYF399S1abc
等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率
16-22-23	0.60%	20-23.1-24.1	0.60%	21-22-26.1	1.30%	21-26-27.1	0.60%	23.1-24-25	0.60%
16-25-27.1	0.60%	20-23.1-25	2.60%	21-23.1	1.30%	21-27.1	0.60%	23.1-24-26.1	0.60%
17.1-19-25	0.60%	20-23-24.1	1.30%	21-23.1-24	0.60%	22.1	0.60%	23-23.1	0.60%
18-23.1-25	0.60%	20-23-25.1	0.60%	21-23.1-25	1.30%	22.1-24	0.60%	23-24.1	1.30%
18-24-25.1	0.60%	20-23-26.1	0.60%	21-23-23.1	2.60%	22.1-25-26	0.60%	23-24.1-25	0.60%
19.1-21-27	0.60%	20-24	0.60%	21-23-24.1	1.30%	22-22.1	0.60%	23-24-24.1	1.30%
19.1-21-28	0.60%	20-24.1-25	0.60%	21-23-24-24.1	0.60%	22-22.1-23	0.60%	23-24-29.1	0.60%
19.1-21-28.2	0.60%	20-24-24.1	1.90%	21-23-25.1	0.60%	22-23	0.60%	24.1	0.60%
19.1-21-29	0.60%	20-24-25.1	0.60%	21-23-26.1	0.60%	22-23.1	1.30%	24.1-26	1.30%
19-20-24.1	0.60%	20-24-26.1	1.30%	21-23-27.1	0.60%	22-23.1-24	1.90%	24.1-26.1	0.60%
19-21.1-23	0.60%	20-24-27.1	1.30%	21-24.1-25	1.30%	22-23-25.1	0.60%	24-24.1-25	0.60%
19-21-23.1	1.30%	20-25.1	0.60%	21-24.1-26	1.30%	22-23-28	0.60%	24-25-26.1	0.60%
19-22.1-23	0.60%	20-25.1-26	0.60%	21-24-24.1	1.30%	22-24.1	0.60%	24-27.1	1.30%
19-23-26.1	0.60%	20-27.1	0.60%	21-24-25.1	2.60%	22-24.1-25	1.30%	25.1-27.1	0.60%
19-24-24.1	0.60%	21	1.30%	21-24-26.1	0.60%	22-24-24.1	0.60%	26.1	3.20%
19-26-26.1	0.60%	21.1	0.60%	21-24-27.1	1.30%	22-24-25.1	2.60%	28.1	0.60%
20.1-22-23	0.60%	21.1-22-24	0.60%	21-25.1-26	0.60%	22-24-27.1	1.30%	30.1	0.60%
20-22.1-25	1.30%	21.1-23-25	0.60%	21-25-25.1	1.90%	22-24-27.1	0.60%	31.1	0.60%
20-22-24.1	0.60%	21-21.1-22-23-23.1	0.60%	21-25-27.1	1.30%	22-26.1-27	0.60%
20-22-28.1	0.60%	21-22.1-23	0.60%	21-25-28.1	0.60%	22-27-27.1	0.60%
20-22-29.1	0.60%	21-22-22.1	1.30%	21-26.1-28	0.60%	23	0.60%
20-23.1-24	1.90%	21-22-23.1	0.60%	21-26-26.1	0.60%	23.1-24.1	1.30%

DYS626		DYF403S1b1		DYS404S1ab		DYF387S1ab		DYF403S1a
等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率
26	1.30%	15.2	2.60%	12-13 13	5.20%	34	0.60%	32-34	0.60%
27	2.60%	16.2	9.70%	12-14	0.60%	34.2-38	0.60%	34-36	0.60%
28	19.50%	17.2	18.20%	12-15	1.30%	34-37	2.60%	34-39	1.30%
29	22.10%	18.2	20.80%	13	18.20%	34-38	1.30%	35.2-42	0.60%
30	16.20%	19.2	18.20%	13-14	20.10%	34-39	3.20%	35-37	0.60%
31	19.50%	20.2	12.30%	13-15	5.80%	34-41	0.60%	35-38	1.90%
32	7.10%	21.2	11.00%	13-16	4.50%	35	1.30%	35-39	1.30%
33	5.80%	22.2	4.50%	14	9.10%	35-37	1.90%	35-41	0.60%
34	5.20%	23.2	1.90%	14-15	7.80%	35-38	14.30%	35-42	0.60%
35	0.60%	24.2	0.60%	14-15-16	0.60%	35-39	7.10%	36	1.30%
DYS449		DYF403S1b2		14-16	3.90%	35-40	0.60%	36-37	1.90%
等位基因	频率	等位基因	频率	14-17	1.90%	36	5.20%	36-38	3.90%
26	1.90%	51.2	2.60%	14-18	0.60%	36-37	6.50%	36-40	1.30%
27	4.50%	52.2	3.20%	15	10.40%	36-38	9.10%	36-42	0.60%
28	7.80%	53.2	2.60%	15-16	5.20%	36-39	5.20%	36-43	0.60%
29	9.70%	54.2	1.30%	15-16-17	0.60%	36-40	3.20%	36-45	0.60%
30	10.40%	55.2	3.90%	16	1.30%	36-41	1.30%	37	1.30%
31	16.20%	56.2	7.80%	16	1.30%	36-42	0.60%	37-38	7.10%
32	13.00%	57.2	9.10%	16-17	1.90%	37	2.60%	37-39	1.90%
33	13.60%	58.2	14.90%	17	0.60%	37-38	8.40%	37-40	2.60%
34	13.60%	59.2	14.30%	DYS526a		37-39	1.90%	37-41	1.30%
35	6.50%	60.2	15.60%	等位基因	频率	37-40	1.30%	37-42	1.90%
36	1.30%	61.2	13.60%	12	11.00%	37-41	0.60%	37-43	2.60%
37	0.60%	62.2	5.80%	13	20.10%	38	5.20%	38	4.50%
38	0.60%	63.2	2.60%	14	29.20%	38-39	1.30%	38.2-41	0.60%
DYS627		64.2	2.60%	15	28.60%	38-40	3.90%	38-39	5.20%
等位基因	频率	DYS612		16	10.40%	38-41	0.60%	38-40	5.20%
16	1.30%	等位基因	频率	17	0.60%	38-42	0.60%	38-41	3.20%
17	3.20%	31	1.30%	DYS526b		39	1.90%	38-42	1.30%
18	15.60%	32	4.50%	等位基因	频率	39-40	3.20%	39	1.90%
19	13.60%	33	4.50%	33	0.60%	40	1.90%	39-40	8.40%
20	20.80%	34	9.70%	34	5.20%	40-41	0.60%	39-41	5.20%
21	16.20%	35	9.70%	35	11.00%	DYS518		39-42	1.90%
21.2	0.60%	36	18.80%	36	15.60%	等位基因	频率	40	5.80%
22	16.90%	37	18.20%	37	33.80%	33	1.30%	40-41	4.50%
23	6.50%	38	12.30%	38	21.40%	34	3.20%	40-43	1.90%
24	4.50%	39	17.50%	39	9.70%	35	12.30%	40-44	0.60%
25	0.60%	40	3.20%	40	2.60%	36	24.70%	41	1.30%
DYS576		DYS547		DYS570		36.2	0.60%	41-42	0.60%
等位基因	频率	等位基因	频率	等位基因	频率	37	16.90%	41-43	3.20%
10	0.60%	42	3.20%	14	2.60%	37.2	0.60%	41-44	0.60%
14	0.60%	43	2.60%	15	4.50%	38	14.90%	41-46	0.60%
15	1.30%	44	3.20%	16	20.80%	39	14.30%	42	1.30%
16	7.80%	45	5.20%	17	21.40%	40	3.90%	42-43	1.30%
17	15.60%	46	7.10%	18	23.40%	41	4.50%	42-44	1.30%
18	39.60%	47	16.90%	19	20.80%	42	0.60%	42-46	0.60%
19	20.10%	48	26.00%	20	5.20%	43	0.60%	43-45	0.60%
20	11.00%	49	14.30%	21	1.30%	44	0.60%
21	2.60%	50	13.00%			46	0.60%
22	0.60%	51	3.90%
		52	4.50%
DYF399S1abc
等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率	等位基因	频率
16-22-23	0.60%	20-23.1-24.1	0.60%	21-22-26.1	1.30%	21-26-27.1	0.60%	23.1-24-25	0.60%
16-25-27.1	0.60%	20-23.1-25	2.60%	21-23.1	1.30%	21-27.1	0.60%	23.1-24-26.1	0.60%
17.1-19-25	0.60%	20-23-24.1	1.30%	21-23.1-24	0.60%	22.1	0.60%	23-23.1	0.60%
18-23.1-25	0.60%	20-23-25.1	0.60%	21-23.1-25	1.30%	22.1-24	0.60%	23-24.1	1.30%
18-24-25.1	0.60%	20-23-26.1	0.60%	21-23-23.1	2.60%	22.1-25-26	0.60%	23-24.1-25	0.60%
19.1-21-27	0.60%	20-24	0.60%	21-23-24.1	1.30%	22-22.1	0.60%	23-24-24.1	1.30%
19.1-21-28	0.60%	20-24.1-25	0.60%	21-23-24-24.1	0.60%	22-22.1-23	0.60%	23-24-29.1	0.60%
19.1-21-28.2	0.60%	20-24-24.1	1.90%	21-23-25.1	0.60%	22-23	0.60%	24.1	0.60%
19.1-21-29	0.60%	20-24-25.1	0.60%	21-23-26.1	0.60%	22-23.1	1.30%	24.1-26	1.30%
19-20-24.1	0.60%	20-24-26.1	1.30%	21-23-27.1	0.60%	22-23.1-24	1.90%	24.1-26.1	0.60%
19-21.1-23	0.60%	20-24-27.1	1.30%	21-24.1-25	1.30%	22-23-25.1	0.60%	24-24.1-25	0.60%
19-21-23.1	1.30%	20-25.1	0.60%	21-24.1-26	1.30%	22-23-28	0.60%	24-25-26.1	0.60%
19-22.1-23	0.60%	20-25.1-26	0.60%	21-24-24.1	1.30%	22-24.1	0.60%	24-27.1	1.30%
19-23-26.1	0.60%	20-27.1	0.60%	21-24-25.1	2.60%	22-24.1-25	1.30%	25.1-27.1	0.60%
19-24-24.1	0.60%	21	1.30%	21-24-26.1	0.60%	22-24-24.1	0.60%	26.1	3.20%
19-26-26.1	0.60%	21.1	0.60%	21-24-27.1	1.30%	22-24-25.1	2.60%	28.1	0.60%
20.1-22-23	0.60%	21.1-22-24	0.60%	21-25.1-26	0.60%	22-24-27.1	1.30%	30.1	0.60%
20-22.1-25	1.30%	21.1-23-25	0.60%	21-25-25.1	1.90%	22-24-27.1	0.60%	31.1	0.60%
20-22-24.1	0.60%	21-21.1-22-23-23.1	0.60%	21-25-27.1	1.30%	22-26.1-27	0.60%
20-22-28.1	0.60%	21-22.1-23	0.60%	21-25-28.1	0.60%	22-27-27.1	0.60%
20-22-29.1	0.60%	21-22-22.1	1.30%	21-26.1-28	0.60%	23	0.60%
20-23.1-24	1.90%	21-22-23.1	0.60%	21-26-26.1	0.60%	23.1-24.1	1.30%

等位基因	D_g值	标准DNA9948基因分型	观察到等位基因范围
DYS526a	0.7746	14	12-17
DYS626	0.8423	28	26-35
DYS526b	0.7959	36	33-40
DYS627	0.8571	22	16-25
DYF403S1b1	0.8563	20.2	15.2-24.2
DYS612	0.8673	37	31-40
DYF403S1b2	0.8972	55.2	51.2-64.2
DYS576	0.7643	16	10-22
DYS570	0.8126	18	14-21
DYS547	0.8580	48	42-52
DYS518	0.8532	38	33-46
DYS449	0.8925	30	26-38
DYF404S1ab	0.8934	12-14	12-17
DYF399S1abc	0.9946	21-22-25.1	16-31.1
DYF387S1ab	0.9444	35-38	34-41
DYF403S1a	0.9688	35-40	32-45

等位基因	D_g值	标准DNA9948基因分型	观察到等位基因范围
DYS526a	0.7746	14	12-17
DYS626	0.8423	28	26-35
DYS526b	0.7959	36	33-40
DYS627	0.8571	22	16-25
DYF403S1b1	0.8563	20.2	15.2-24.2
DYS612	0.8673	37	31-40
DYF403S1b2	0.8972	55.2	51.2-64.2
DYS576	0.7643	16	10-22
DYS570	0.8126	18	14-21
DYS547	0.8580	48	42-52
DYS518	0.8532	38	33-46
DYS449	0.8925	30	26-38
DYF404S1ab	0.8934	12-14	12-17
DYF399S1abc	0.9946	21-22-25.1	16-31.1
DYF387S1ab	0.9444	35-38	34-41
DYF403S1a	0.9688	35-40	32-45