Comparison and summary of ancient DNA extraction technology

doi:10.16359/j.cnki.cn11-1963/q.2020.0062

Abstract

Abstract:

There are many methods to extract ancient DNA from ancient raw materials. Generally, the research of ancient DNA is subject to factors such as serious degradation, while the low content of endogenous ancient DNA, the high content of microbial and modern human DNA. Whether we can successfully obtain reliable and sufficient endogenous ancient DNA has always been a practical difficulty and challenge in the field of ancient DNA research. The most direct and convenient strategy to eliminate the pollution effectively is in the procedure of ancient DNA extraction. This paper summarized the common methods of ancient DNA extraction to remove exogenous pollutants. We compared and analyzed the advantages and disadvantages of each method. This paper also introduced the time commonly used in the bone lysis step and suggested the best incubation time was 4 days at room temperature by exploring the effect of different lysis time on the recovery efficiency of ancient DNA. At the same time, we surveyed the representative methods of ancient DNA purification and the performance in the application. Our summary and experience could provide reference information for researchers in the field of ancient DNA research.

Key words: Ancient DNA, Extraction technology, Pollution prevention and control

CLC Number:

：Q986

ZHAO Jing, WANG Chuanchao. Comparison and summary of ancient DNA extraction technology[J]. Acta Anthropologica Sinica, 2020, 39(04): 706-716.

Figures/Tables 1

References 69

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