The jades excavated from Yin Ruins are significant for exploring the culture and technology in late Shang Dynasty. In this paper, we used silica glue to duplicate some perforated jades’ micro-wears from Yin Ruins. The optical microscope and scanning electron microscope were used to observe and analysis the micro-wears systematically. The results show that there were five different micro-wears, including circular grooves, parallel lengthwise grooves, curved rim, oval pits and irregular lengthwise lines. At the same time, according to the characteristics of micro-wears morphology, distribution, integrity and lamination relationship, it is believed that the formation was mainly related to processing and using. This study points out that based on the micro-wears characteristics inside the jade tunnel, it is expected to establish the relationship between micro-wears and process or use; and through the discussion of micro-wears’ formation, it can provide important scientific evidence for the processing, reworking and using of jade, thus profoundly interpreting the cultural connotation of ancient jade.