In order to explore the effects of different ball games on the bone density and directional asymmetry of upper and lower limbs, 210 subjects were selected, whose age is 20.12±1.02 years old, who are right handness unddivided into 7 groups: basketball group, volleyball group, football group, badminton group, table tennis group, tennis group and control group, 30 persons per group, each group had a balanced sex ratio, similar height, normal body fat and body weight. The training years of each ball group were 6-8 years old, while the control group had no professional training years. Bone mineral density and Directional Asymmetry (DA) were measured. The results showed that the Upper Limb BMD of basketball group, volleyball group, badminton group, table tennis group and tennis group was significantly higher than that of the left Bone mineral density and DA of upper and lower limbs were significantly different among different groups. The results also showed that the order of bone density of left upper limb was volleyball group > football group > table tennis group, tennis group and badminton group > control group, basketball group > table tennis group, tennis group and badminton group > control group. The order of bone density of right upper limb was tennis group > table tennis group, volleyball group and basketball group > football group > control group, table tennis group > badminton group> football group > control group. The order of bone density of left lower limb was football group > basketball group, volleyball group, badminton group, table tennis group and tennis group > control group. The order of bone density of right lower limb was football group > volleyball group, basketball group, tennis group, badminton group and table tennis group > control group, volleyball group > badminton group and table tennis group > control group The order of DA is tennis group > table tennis group, badminton group, basketball group and volleyball group, table tennis group > basketball group and volleyball group. The results suggest that ball games can improve the bone density of upper and lower limbs and cause the asymmetry of its direction. The reason may be related to the mechanical stimulation to the bone.